› 7pw1

X-ray diffraction
1.5Å resolution

Crystal structure of ancestral haloalkane dehalogenase AncLinB-DmbA

Released:
DOI: 10.2210/pdb7pw1/pdb
PDB entry 7pw1 coloured by chain, front view.
PDB entry 7pw1 coloured by chain, side view.
PDB entry 7pw1 coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Structural Analysis of the Ancestral Haloalkane Dehalogenase AncLinB-DmbA.
Mazur A, Grinkevich P, Chaloupkova R, Havlickova P, Kascakova B, Kuty M, Damborsky J, Kuta Smatanova I, Prudnikova T
Int J Mol Sci 22 (2021)
PMID: 34769421

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-168637 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Haloalkane dehalogenase Chain: A
Molecule details ›
Chain: A
Length: 296 amino acids
Theoretical weight: 33.35 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

3 bound ligands:
Ligand CL 1 x CL
Ligand NA 4 x NA
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P43212
Unit cell:
a: 68.897Å b: 68.897Å c: 156.259Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.14 0.138 0.174
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

Structural Analysis of the Ancestral Haloalkane Dehalogenase AncLinB-DmbA.
Mazur et al. (2021)