› 7q21

Electron Microscopy
3Å resolution

III2-IV2 respiratory supercomplex from Corynebacterium glutamicum

Released:
DOI: 10.2210/pdb7q21/pdb
PDB entry 7q21 coloured by chain, front view.
PDB entry 7q21 coloured by chain, side view.
PDB entry 7q21 coloured by chain, top view.
Source organism: Corynebacterium glutamicum ATCC 13032
Primary publication:
The respiratory supercomplex from C. glutamicum.
Moe A, Kovalova T, Król S, Yanofsky DJ, Bott M, Sjöstrand D, Rubinstein JL, Högbom M, Brzezinski P
Structure 30 338-349.e3 (2022)
PMID: 34910901
Related structures: EMD-13777

Function and Biology Details

Reactions catalysed: 
4 ferrocytochrome c + O(2) + 8 H(+)(Side 1) = 4 ferricytochrome c + 4 H(2)O(Side 2)
Quinol + 2 ferricytochrome c(Side 2) = quinone + 2 ferrocytochrome c(Side 2) + 2 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 26-mer (preferred)
PDBe Complex ID:
PDB-CPX-168650 (preferred)
Entry contents:
14 distinct polypeptide molecules
Macromolecules (14 distinct):
Co-purified unknown transmembrane helices built as polyALA (AscD) Chains: Y, y
Molecule details ›
Chains: Y, y
Length: 96 amino acids
Theoretical weight: 8.19 KDa
Source organism: Corynebacterium glutamicum ATCC 13032
Expression system: Corynebacterium glutamicum ATCC 13032
Cytochrome c oxidase subunit 3 Chains: E, e
Molecule details ›
Chains: E, e
Length: 205 amino acids
Theoretical weight: 22.46 KDa
Source organism: Corynebacterium glutamicum ATCC 13032
Expression system: Corynebacterium glutamicum ATCC 13032
UniProt:
  • Canonical: Q9AEL8 (Residues: 1-205; Coverage: 100%)
Gene names: Cgl2192, cg2406, ctaE
Sequence domains: Cytochrome c oxidase subunit III
Uncharacterized membrane protein Cgl2017/cg2211 Chains: H, h
Molecule details ›
Chains: H, h
Length: 147 amino acids
Theoretical weight: 16.39 KDa
Source organism: Corynebacterium glutamicum ATCC 13032
Expression system: Corynebacterium glutamicum ATCC 13032
UniProt:
  • Canonical: Q8NP09 (Residues: 1-147; Coverage: 100%)
Gene names: Cgl2017, cg2211
Sequence domains: Protein of unknown function (DUF2631)
Cytochrome c oxidase polypeptide 4 Chains: F, f
Molecule details ›
Chains: F, f
Length: 143 amino acids
Theoretical weight: 15.56 KDa
Source organism: Corynebacterium glutamicum ATCC 13032
Expression system: Corynebacterium glutamicum ATCC 13032
UniProt:
  • Canonical: Q8NNK3 (Residues: 1-143; Coverage: 100%)
Gene names: Cgl2194, cg2408, ctaF
Sequence domains: Cytochrome c oxidase subunit IV
Cytochrome c oxidase subunit 2 Chains: G, g
Molecule details ›
Chains: G, g
Length: 359 amino acids
Theoretical weight: 39.67 KDa
Source organism: Corynebacterium glutamicum ATCC 13032
Expression system: Corynebacterium glutamicum ATCC 13032
UniProt:
  • Canonical: Q8NNK2 (Residues: 1-359; Coverage: 100%)
Gene names: Cgl2195, cg2409, ctaC
Sequence domains: Cytochrome C oxidase subunit II, periplasmic domain
Cytochrome c oxidase subunit 1 Chains: D, d
Molecule details ›
Chains: D, d
Length: 594 amino acids
Theoretical weight: 66.34 KDa
Source organism: Corynebacterium glutamicum ATCC 13032
Expression system: Corynebacterium glutamicum ATCC 13032
UniProt:
  • Canonical: Q79VD7 (Residues: 1-584; Coverage: 100%)
Gene names: Cgl2523, cg2780, ctaD
Sequence domains: Cytochrome C and Quinol oxidase polypeptide I
Cytochrome bc1 complex Rieske iron-sulfur subunit Chains: A, a
Molecule details ›
Chains: A, a
Length: 408 amino acids
Theoretical weight: 45.23 KDa
Source organism: Corynebacterium glutamicum ATCC 13032
Expression system: Corynebacterium glutamicum ATCC 13032
UniProt:
  • Canonical: Q79VE8 (Residues: 1-408; Coverage: 100%)
Gene names: Cgl2190, cg2404, qcrA
Sequence domains:
P29 Chains: L, l
Molecule details ›
Chains: L, l
Length: 194 amino acids
Theoretical weight: 20.03 KDa
Source organism: Corynebacterium glutamicum ATCC 13032
Expression system: Corynebacterium glutamicum ATCC 13032
UniProt:
  • Canonical: Q8NMB4 (Residues: 1-194; Coverage: 100%)
Gene names: Cgl2664, cg2949
Cytochrome bc1 complex cytochrome b subunit Chains: B, b
Molecule details ›
Chains: B, b
Length: 539 amino acids
Theoretical weight: 59.86 KDa
Source organism: Corynebacterium glutamicum ATCC 13032
Expression system: Corynebacterium glutamicum ATCC 13032
UniProt:
  • Canonical: Q79VE9 (Residues: 1-539; Coverage: 100%)
Gene names: Cgl2189, cg2403, qcrB
Sequence domains: Cytochrome b(N-terminal)/b6/petB
Cytochrome bc1 complex cytochrome c subunit Chains: C, c
Molecule details ›
Chains: C, c
Length: 283 amino acids
Theoretical weight: 29.9 KDa
Source organism: Corynebacterium glutamicum ATCC 13032
Expression system: Corynebacterium glutamicum ATCC 13032
UniProt:
  • Canonical: Q8NNK5 (Residues: 1-283; Coverage: 100%)
Gene names: Cgl2191, cg2405, qcrC
Sequence domains:
Co-purified unknown peptide built as polyALA (AscE) Chain: X
Molecule details ›
Chain: X
Length: 21 amino acids
Theoretical weight: 1.82 KDa
Source organism: Corynebacterium glutamicum ATCC 13032
Expression system: Corynebacterium glutamicum ATCC 13032
Co-purified unknown peptide built as polyALA (AscE) Chain: x
Molecule details ›
Chain: x
Length: 22 amino acids
Theoretical weight: 1.91 KDa
Source organism: Corynebacterium glutamicum ATCC 13032
Expression system: Corynebacterium glutamicum ATCC 13032
Actinobacterial supercomplex, subunit C (AscC) Chains: V, v
Molecule details ›
Chains: V, v
Length: 73 amino acids
Theoretical weight: 8.37 KDa
Source organism: Corynebacterium glutamicum ATCC 13032
Expression system: Corynebacterium glutamicum ATCC 13032
UniProt:
  • Canonical: Q8NS61 (Residues: 1-73; Coverage: 100%)
Gene name: Cgl0818
Hypothetical membrane protein Chains: K, k
Molecule details ›
Chains: K, k
Length: 65 amino acids
Theoretical weight: 6.63 KDa
Source organism: Corynebacterium glutamicum ATCC 13032
Expression system: Corynebacterium glutamicum ATCC 13032
UniProt:
  • Canonical: Q8NSJ8 (Residues: 1-65; Coverage: 100%)
Gene name: Cgl0673

Ligands and Environments

15 bound ligands:
Ligand CDL 14 x CDL
Ligand 7PH 16 x 7PH
Ligand TRD 18 x TRD
Ligand 9XX 4 x 9XX
Ligand TWT 2 x TWT
Ligand CU 6 x CU
Ligand MG 2 x MG
Ligand HAS 4 x HAS
Ligand CA 2 x CA
Ligand FES 2 x FES
Ligand MQ9 6 x MQ9
Ligand 9YF 4 x 9YF
Ligand PLM 4 x PLM
Ligand HEM 4 x HEM
Ligand HEC 4 x HEC
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-13777
Expression system: Corynebacterium glutamicum ATCC 13032

Quick links

Citations

2 review citations

Bioenergetic Aspects of Mitochondrial Actions of Thyroid Hormones.
Cioffi et al. (2022)
1 more

245 mentions without citation

The ADAM metalloproteinases.
Edwards et al. (2008)
244 more