› 7q4j

X-ray diffraction
1.91Å resolution

A thermostable lipase from Thermoanaerobacter thermohydrosulfuricus in complex a monoacylglycerol intermediate

Released:
DOI: 10.2210/pdb7q4j/pdb
PDB entry 7q4j coloured by chain, front view.
PDB entry 7q4j coloured by chain, side view.
PDB entry 7q4j coloured by chain, top view.
Source organism: Thermoanaerobacter thermohydrosulfuricus
Entry authors: Pinotsis N, Wilmanns M

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-232539 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine aminopeptidase S33 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 259 amino acids
Theoretical weight: 29.96 KDa
Source organism: Thermoanaerobacter thermohydrosulfuricus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1G7VV58 (Residues: 1-259; Coverage: 100%)
Gene name: SAMN04244560_02687
Sequence domains: Serine aminopeptidase, S33
Serine aminopeptidase S33 domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 259 amino acids
Theoretical weight: 30.04 KDa
Source organism: Thermoanaerobacter thermohydrosulfuricus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1G7VV58 (Residues: 1-259; Coverage: 100%)
Gene name: SAMN04244560_02687
Sequence domains: Serine aminopeptidase, S33

Ligands and Environments

7 bound ligands:
Ligand STE 2 x STE
Ligand OCT 2 x OCT
Ligand GOL 7 x GOL
Ligand SO4 4 x SO4
Ligand CL 1 x CL
Ligand DD9 1 x DD9
Ligand D10 1 x D10
3 modified residues:
Ligand MSE 22 x MSE
Ligand MLY 16 x MLY
Ligand MLZ 10 x MLZ

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P43212
Unit cell:
a: 137.526Å b: 137.526Å c: 67.781Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.163 0.194
Expression system: Escherichia coli

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