› 7q7q

X-ray diffraction
2.25Å resolution

LIPIDIC CUBIC PHASE SERIAL FEMTOSECOND CRYSTALLOGRAPHY STRUCTURE OF A PHOTOSYNTHETIC REACTION CENTRE

Released:
DOI: 10.2210/pdb7q7q/pdb
Model geometry
Fit model/data
PDB entry 7q7q coloured by chain, front view.
PDB entry 7q7q coloured by chain, side view.
PDB entry 7q7q coloured by chain, top view.
Source organism: Blastochloris viridis
Primary publication:
Lipidic cubic phase serial femtosecond crystallography structure of a photosynthetic reaction centre.
Båth P, Banacore A, Börjesson P, Bosman R, Wickstrand C, Safari C, Dods R, Ghosh S, Dahl P, Ortolani G, Björg Ulfarsdottir T, Hammarin G, García Bonete MJ, Vallejos A, Ostojić L, Edlund P, Linse JB, Andersson R, Nango E, Owada S, Tanaka R, Tono K, Joti Y, Nureki O, Luo F, James D, Nass K, Johnson PJM, Knopp G, Ozerov D, Cirelli C, Milne C, Iwata S, Brändén G, Neutze R
OpenAccess logo Acta Crystallogr D Struct Biol 78 698-708 (2022)
PMID: 35647917

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-138851 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Photosynthetic reaction center cytochrome c subunit Chain: CCC
Molecule details ›
Chain: CCC
Length: 336 amino acids
Theoretical weight: 37.45 KDa
Source organism: Blastochloris viridis
Expression system: Blastochloris viridis
UniProt:
  • Canonical: P07173 (Residues: 21-356; Coverage: 100%)
Gene names: BVIRIDIS_00500, cytC, pufC
Sequence domains: Photosynthetic reaction centre cytochrome C subunit
Reaction center protein H chain Chain: HHH
Molecule details ›
Chain: HHH
Length: 258 amino acids
Theoretical weight: 28.56 KDa
Source organism: Blastochloris viridis
UniProt:
  • Canonical: P06008 (Residues: 1-258; Coverage: 100%)
Gene name: puhA
Sequence domains:
Reaction center protein L chain Chain: LLL
Reaction center protein M chain Chain: MMM

Ligands and Environments

12 bound ligands:
Ligand HEC 4 x HEC
Ligand DGA 1 x DGA
Ligand LDA 7 x LDA
Ligand SO4 5 x SO4
Ligand BCB 4 x BCB
Ligand BPB 2 x BPB
Ligand HTO 8 x HTO
Ligand UQ2 2 x UQ2
Ligand FE2 1 x FE2
Ligand MQ9 1 x MQ9
Ligand NS5 1 x NS5
Ligand OLC 2 x OLC
1 modified residue:
Ligand FME 1 x FME

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SwissFEL ARAMIS BEAMLINE ESA
Spacegroup: P21212
Unit cell:
a: 84.66Å b: 125.13Å c: 182.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.168 0.203
Expression system: Blastochloris viridis

Quick links

Citations

1 review citation

Bridging the microscopic divide: a comprehensive overview of micro-crystallization and in vivo crystallography.
Chavas et al. (2024)
5 other articles

2 mentions without citation

Lipidic cubic phase serial femtosecond crystallography structure of a photosynthetic reaction centre.
Båth et al. (2022)
1 more