› 7qco

Electron Microscopy
3.7Å resolution

The structure of Photosystem I tetramer from Chroococcidiopsis TS-821, a thermophilic, unicellular, non-heterocyst-forming cyanobacterium

Released:
DOI: 10.2210/pdb7qco/pdb
PDB entry 7qco coloured by chain, front view.
PDB entry 7qco coloured by chain, side view.
PDB entry 7qco coloured by chain, top view.
Source organism: Chroococcidiopsis sp. TS-821
Primary publication:
Cryo-EM structure of a tetrameric photosystem I from Chroococcidiopsis TS-821, a thermophilic, unicellular, non-heterocyst-forming cyanobacterium.
Semchonok DA, Mondal J, Cooper CJ, Schlum K, Li M, Amin M, Sorzano COS, Ramírez-Aportela E, Kastritis PL, Boekema EJ, Guskov A, Bruce BD
Plant Commun 3 100248 (2022)
PMID: 35059628
Related structures: EMD-13898

Function and Biology Details

Reaction catalysed: 
Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 44-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104493 (preferred)
Entry contents:
11 distinct polypeptide molecules
Macromolecules (11 distinct):
Photosystem I P700 chlorophyll a apoprotein A1 Chains: A, E, a, e
Molecule details ›
Chains: A, E, a, e
Length: 752 amino acids
Theoretical weight: 83.35 KDa
Source organism: Chroococcidiopsis sp. TS-821
UniProt:
  • Canonical: A0A2S6VGM5 (Residues: 1-752; Coverage: 100%)
Gene names: B1A85_03120, psaA
Sequence domains: Photosystem I psaA/psaB protein
Photosystem I P700 chlorophyll a apoprotein A2 Chains: B, G, b, g
Molecule details ›
Chains: B, G, b, g
Length: 737 amino acids
Theoretical weight: 82.66 KDa
Source organism: Chroococcidiopsis sp. TS-821
UniProt:
  • Canonical: A0A2S6VGS2 (Residues: 1-737; Coverage: 100%)
Gene names: B1A85_03115, psaB
Sequence domains: Photosystem I psaA/psaB protein
Photosystem I iron-sulfur center Chains: C, H, c, h
Molecule details ›
Chains: C, H, c, h
Length: 82 amino acids
Theoretical weight: 8.94 KDa
Source organism: Chroococcidiopsis sp. TS-821
UniProt:
  • Canonical: A0A2S6VD54 (Residues: 1-82; Coverage: 100%)
Gene names: B1A85_08600, psaC
Sequence domains: 4Fe-4S dicluster domain
Photosystem I reaction center subunit II Chains: D, N, d, n
Molecule details ›
Chains: D, N, d, n
Length: 168 amino acids
Theoretical weight: 18.52 KDa
Source organism: Chroococcidiopsis sp. TS-821
UniProt:
  • Canonical: A0A2S6VI21 (Residues: 1-168; Coverage: 100%)
Gene name: B1A85_06405
Sequence domains: PsaD
Photosystem I reaction center subunit IV Chains: V, W, v, w
Molecule details ›
Chains: V, W, v, w
Length: 126 amino acids
Theoretical weight: 13.55 KDa
Source organism: Chroococcidiopsis sp. TS-821
UniProt:
  • Canonical: A0A2S6VHU0 (Residues: 1-126; Coverage: 100%)
Gene names: B1A85_05935, psaE
Sequence domains: Photosystem I reaction centre subunit IV / PsaE
Photosystem I reaction center subunit III Chains: F, O, f, o
Molecule details ›
Chains: F, O, f, o
Length: 164 amino acids
Theoretical weight: 18.13 KDa
Source organism: Chroococcidiopsis sp. TS-821
UniProt:
  • Canonical: A0A2S6VDZ1 (Residues: 1-164; Coverage: 100%)
Gene name: B1A85_10140
Sequence domains: Photosystem I reaction centre subunit III
Photosystem I reaction center subunit VIII Chains: I, P, i, p
Molecule details ›
Chains: I, P, i, p
Length: 39 amino acids
Theoretical weight: 4.19 KDa
Source organism: Chroococcidiopsis sp. TS-821
UniProt:
  • Canonical: A0A2S6VDT4 (Residues: 1-39; Coverage: 100%)
Gene names: B1A85_09815, psaI
Sequence domains: Photosystem I reaction centre subunit VIII
Photosystem I reaction center subunit IX Chains: J, Q, j, q
Molecule details ›
Chains: J, Q, j, q
Length: 49 amino acids
Theoretical weight: 5.76 KDa
Source organism: Chroococcidiopsis sp. TS-821
UniProt:
  • Canonical: V5JYA2 (Residues: 1-49; Coverage: 100%)
Gene names: B1A85_10135, psaJ
Sequence domains: Photosystem I reaction centre subunit IX / PsaJ
Photosystem I reaction center subunit PsaK Chains: K, R, k, r
Molecule details ›
Chains: K, R, k, r
Length: 93 amino acids
Theoretical weight: 9.75 KDa
Source organism: Chroococcidiopsis sp. TS-821
UniProt:
  • Canonical: A0A2S6V467 (Residues: 1-93; Coverage: 100%)
Gene name: B1A85_20020
Sequence domains: Photosystem I psaG / psaK
Photosystem I reaction center subunit XI Chains: L, S, l, s
Molecule details ›
Chains: L, S, l, s
Length: 172 amino acids
Theoretical weight: 18.5 KDa
Source organism: Chroococcidiopsis sp. TS-821
UniProt:
  • Canonical: V5JYE0 (Residues: 1-172; Coverage: 100%)
Gene names: B1A85_10130, psaL
Sequence domains: Photosystem I reaction centre subunit XI
Photosystem I reaction center subunit XII Chains: M, T, m, t
Molecule details ›
Chains: M, T, m, t
Length: 31 amino acids
Theoretical weight: 3.38 KDa
Source organism: Chroococcidiopsis sp. TS-821
UniProt:
  • Canonical: A0A2S6V6A8 (Residues: 1-31; Coverage: 100%)
Gene names: B1A85_18180, psaM
Sequence domains: Photosystem I protein M (PsaM)

Ligands and Environments

5 bound ligands:
Ligand LHG 5 x LHG
Ligand CLA 359 x CLA
Ligand PQN 8 x PQN
Ligand BCR 78 x BCR
Ligand SF4 12 x SF4
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-13898

Quick links

Citations

7 citation in other articles

Alternatives to Styrene- and Diisobutylene-Based Copolymers for Membrane Protein Solubilization via Nanodisc Formation.
Workman et al. (2023)
6 more