› 7qdt

X-ray diffraction
3Å resolution

Crystal structure of a mutant (P393GX) Thyroid Receptor Alpha ligand binding domain designed to model dominant negative human mutations.

Released:
DOI: 10.2210/pdb7qdt/pdb
PDB entry 7qdt coloured by chain, front view.
PDB entry 7qdt coloured by chain, side view.
PDB entry 7qdt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure-Guided Approach to Relieving Transcriptional Repression in Resistance to Thyroid Hormone α.
Romartinez-Alonso B, Agostini M, Jones H, McLellan J, Sood DE, Tomkinson N, Marelli F, Gentile I, Visser WE, Schoenmakers E, Fairall L, Privalsky M, Moran C, Persani L, Chatterjee K, Schwabe JWR
Mol Cell Biol 42 e0036321 (2022)
PMID: 34871063

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145479 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thyroid hormone receptor alpha Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10827 (Residues: 156-370; Coverage: 44%)
Gene names: EAR7, ERBA1, NR1A1, THRA, THRA1, THRA2
Sequence domains: Ligand-binding domain of nuclear hormone receptor

Ligands and Environments

1 bound ligand:
Ligand T3 1 x T3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P6422
Unit cell:
a: 143.328Å b: 143.328Å c: 88.502Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.183 0.228
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Long-term follow-up results and treatment outcomes of children and adults with resistance to thyroid hormone alpha.
Erbaş et al. (2023)
1 more

1 mention without citation

Structure-Guided Approach to Relieving Transcriptional Repression in Resistance to Thyroid Hormone α.
Romartinez-Alonso et al. (2022)