› 7qfn

X-ray diffraction
2.62Å resolution

Function and Biology Details

Reaction catalysed: 
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169779 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA topoisomerase 2-beta Chain: A
Molecule details ›
Chain: A
Length: 403 amino acids
Theoretical weight: 45.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02880 (Residues: 50-449; Coverage: 25%)
Gene name: TOP2B
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand ADP 1 x ADP
Ligand SO4 2 x SO4
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P321
Unit cell:
a: 103.021Å b: 103.021Å c: 66.693Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.199 0.259
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Cell cycle responses to Topoisomerase II inhibition: Molecular mechanisms and clinical implications.
Soliman et al. (2023)
2 other articles

1 mention without citation

A comprehensive structural analysis of the ATPase domain of human DNA topoisomerase II beta bound to AMPPNP, ADP, and the bisdioxopiperazine, ICRF193.
Ling et al. (2022)