› 7qim

Electron Microscopy
4.5Å resolution

In situ structure of nebulin bound to actin filament in skeletal sarcomere

Released:
DOI: 10.2210/pdb7qim/pdb
PDB entry 7qim coloured by chain, front view.
PDB entry 7qim coloured by chain, side view.
PDB entry 7qim coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structures from intact myofibrils reveal mechanism of thin filament regulation through nebulin.
Wang Z, Grange M, Pospich S, Wagner T, Kho AL, Gautel M, Raunser S
Science 375 eabn1934 (2022)
PMID: 35175800
Related structures: EMD-13990

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159300 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Actin, alpha skeletal muscle Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 377 amino acids
Theoretical weight: 42.11 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P68134 (Residues: 1-377; Coverage: 100%)
Gene names: Acta, Acta1
Sequence domains: Actin
nebulin (mouse) Chain: F
Molecule details ›
Chain: F
Length: 105 amino acids
Theoretical weight: 9.19 KDa
Source organism: Mus musculus
nebulin (mouse) Chain: G
Molecule details ›
Chain: G
Length: 70 amino acids
Theoretical weight: 6.13 KDa
Source organism: Mus musculus

Ligands and Environments

2 bound ligands:
Ligand ADP 5 x ADP
Ligand MG 5 x MG
1 modified residue:
Ligand HIC 5 x HIC

Experiments and Validation Details

Entry percentile scores
Resolution: 4.5Å
Relevant EMDB volumes: EMD-13990

Quick links

Citations

5 review citations

Charting the molecular landscape of the cell.
Ochner et al. (2023)
4 more

3 mentions without citation

The Mechanisms of Thin Filament Assembly and Length Regulation in Muscles.
Szikora et al. (2022)
2 more