7qin

Electron Microscopy
6.6Å resolution

In situ structure of actomyosin complex in skeletal sarcomere

Released:
Source organism: Mus musculus
Related structures: EMD-13991

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tridecamer (preferred)
PDBe Complex ID:
PDB-CPX-159301 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Actin, alpha skeletal muscle Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 371 amino acids
Theoretical weight: 41.39 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P68134 (Residues: 7-377; Coverage: 98%)
Gene names: Acta, Acta1
Sequence domains: Actin
nebulin (mouse) Chain: F
Molecule details ›
Chain: F
Length: 105 amino acids
Theoretical weight: 9.19 KDa
Source organism: Mus musculus
nebulin (mouse) Chain: G
Molecule details ›
Chain: G
Length: 70 amino acids
Theoretical weight: 6.13 KDa
Source organism: Mus musculus
tropomyosin, alpha-1 (mouse) Chain: H
Molecule details ›
Chain: H
Length: 117 amino acids
Theoretical weight: 9.98 KDa
Source organism: Mus musculus
tropomyosin, alpha-1 (mouse) Chain: I
Molecule details ›
Chain: I
Length: 118 amino acids
Theoretical weight: 10.06 KDa
Source organism: Mus musculus
tropomyosin, alpha-1 (mouse) Chains: J, K
Molecule details ›
Chains: J, K
Length: 88 amino acids
Theoretical weight: 7.51 KDa
Source organism: Mus musculus
Myosin-4 Chains: L, M
Molecule details ›
Chains: L, M
Length: 848 amino acids
Theoretical weight: 96.41 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q5SX39 (Residues: 1-848; Coverage: 44%)
Gene name: Myh4
Sequence domains:

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution: 6.6Å
Relevant EMDB volumes: EMD-13991