› 7qjm

X-ray diffraction
2.19Å resolution

Crystal structure of an alpha/beta-hydrolase enzyme from Chloroflexus sp. MS-G (202)

Released:
DOI: 10.2210/pdb7qjm/pdb
PDB entry 7qjm coloured by chain, front view.
PDB entry 7qjm coloured by chain, side view.
PDB entry 7qjm coloured by chain, top view.
Source organism: Chloroflexus sp. MS-G
Primary publication:
Sourcing thermotolerant poly(ethylene terephthalate) hydrolase scaffolds from natural diversity
Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, Beckham GT, McGeehan JE
Nat Commun 13 7850- (2022)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-241580 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
alpha/beta-hydrolase (202) Chains: A, B
Molecule details ›
Chains: A, B
Length: 388 amino acids
Theoretical weight: 42.32 KDa
Source organism: Chloroflexus sp. MS-G
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: I222
Unit cell:
a: 90.68Å b: 124.91Å c: 169.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.216 0.244
Expression system: Escherichia coli BL21(DE3)

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