› 7qjs

X-ray diffraction
1.43Å resolution

Function and Biology Details

Reactions catalysed: 
(Ethylene terephthalate)(n) + H(2)O = (ethylene terephthalate)(n-1) + ethylene terephthalate
Cutin + H(2)O = cutin monomers
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-222591 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cutinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 270 amino acids
Theoretical weight: 29.63 KDa
Source organism: Thermobifida fusca YX
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q47RJ7 (Residues: 59-319; Coverage: 94%)
Gene name: Tfu_0882
Sequence domains: Platelet-activating factor acetylhydrolase, isoform II

Ligands and Environments

3 bound ligands:
Ligand GOL 2 x GOL
Ligand PG4 1 x PG4
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P21
Unit cell:
a: 36.164Å b: 150.212Å c: 43.396Å
α: 90° β: 92.509° γ: 90°
R-values:
R R work R free
0.183 0.182 0.216
Expression system: Escherichia coli BL21(DE3)

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