7qng

X-ray diffraction
2.7Å resolution

Structure of a MHC I-Tapasin-ERp57 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127516 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Tapasin Chain: A
Molecule details ›
Chain: A
Length: 419 amino acids
Theoretical weight: 44.88 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O15533 (Residues: 1-401; Coverage: 89%)
Gene names: NGS17, TAPA, TAPBP
Sequence domains: Immunoglobulin C1-set domain
Protein disulfide-isomerase A3 Chain: B
Molecule details ›
Chain: B
Length: 505 amino acids
Theoretical weight: 56.83 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P30101 (Residues: 1-505; Coverage: 100%)
Gene names: ERP57, ERP60, GRP58, PDIA3
Sequence domains:
H-2 class I histocompatibility antigen, D-B alpha chain Chain: C
Molecule details ›
Chain: C
Length: 277 amino acids
Theoretical weight: 32.22 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01899 (Residues: 25-300; Coverage: 82%)
Gene name: H2-D1
Sequence domains:
Beta-2-microglobulin Chain: D
Molecule details ›
Chain: D
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Unit cell:
a: 74.76Å b: 168.53Å c: 187.05Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.229
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli