› 7qnm

X-ray diffraction
2.73Å resolution

Crystallization and structural analyses of ZgHAD, a L-2-haloacid dehalogenase from the marine Flavobacterium Zobellia galactanivorans

Released:
DOI: 10.2210/pdb7qnm/pdb
PDB entry 7qnm coloured by chain, front view.
PDB entry 7qnm coloured by chain, side view.
PDB entry 7qnm coloured by chain, top view.
Source organism: Zobellia galactanivorans
Primary publication:
X-ray structure and mechanism of ZgHAD, a L-2-haloacid dehalogenase from the marine Flavobacterium Zobellia galactanivorans.
Grigorian E, Roret T, Czjzek M, Leblanc C, Delage L
Protein Sci e4540 (2022)
PMID: 36502283

Function and Biology Details

Reaction catalysed: 
(S)-2-haloacid + H(2)O = (R)-2-hydroxyacid + halide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123849 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(S)-2-haloacid dehalogenase Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z
Length: 227 amino acids
Theoretical weight: 25.15 KDa
Source organism: Zobellia galactanivorans
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: G0L7V6 (Residues: 1-227; Coverage: 100%)
Gene names: dhlB, zobellia_4183
Sequence domains: haloacid dehalogenase-like hydrolase

Ligands and Environments

1 bound ligand:
Ligand PO4 26 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P21
Unit cell:
a: 76.165Å b: 132.789Å c: 275.698Å
α: 90° β: 92.31° γ: 90°
R-values:
R R work R free
0.209 0.206 0.254
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

1 mention without citation

X-ray structure and mechanism of ZgHAD, a l-2-haloacid dehalogenase from the marine Flavobacterium Zobellia galactanivorans.
Grigorian et al. (2023)