› 7qoa

X-ray diffraction
2.4Å resolution

Structure of CodB, a cytosine transporter in an outward-facing conformation

Released:
DOI: 10.2210/pdb7qoa/pdb
PDB entry 7qoa coloured by chain, front view.
PDB entry 7qoa coloured by chain, side view.
PDB entry 7qoa coloured by chain, top view.
Source organism: Proteus vulgaris
Primary publication:
Structure of cytosine transport protein CodB provides insight into nucleobase-cation symporter 1 mechanism.
Hatton CE, Brotherton DH, Spencer M, Cameron AD
EMBO J e110527 (2022)
PMID: 35775318

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-241581 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytosine permease Chains: A, B
Molecule details ›
Chains: A, B
Length: 423 amino acids
Theoretical weight: 44.19 KDa
Source organism: Proteus vulgaris
Expression system: Escherichia coli BL21

Ligands and Environments

5 bound ligands:
Ligand CYT 2 x CYT
Ligand PEF 1 x PEF
Ligand LMT 1 x LMT
Ligand A6L 5 x A6L
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: C2221
Unit cell:
a: 108.246Å b: 209.027Å c: 102.452Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.218 0.265
Expression system: Escherichia coli BL21

Quick links

Citations

2 citation in other articles

A Methylotrophic Bacterium Growing with the Antidiabetic Drug Metformin as Its Sole Carbon, Nitrogen and Energy Source.
Chaignaud et al. (2022)
1 more

1 mention without citation

Structure of cytosine transport protein CodB provides insight into nucleobase-cation symporter 1 mechanism.
Hatton et al. (2022)