› 7qpj

X-ray diffraction
1.54Å resolution

Crystal structure of engineered TCR (756) complexed to HLA-A*02:01 presenting MAGE-A10 9-mer peptide

Released:
DOI: 10.2210/pdb7qpj/pdb
PDB entry 7qpj coloured by chain, front view.
PDB entry 7qpj coloured by chain, side view.
PDB entry 7qpj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into engineering a T-cell receptor targeting MAGE-A10 with higher affinity and specificity for cancer immunotherapy.
Simister PC, Border EC, Vieira JF, Pumphrey NJ
J Immunother Cancer 10 (2022)
PMID: 35851311

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-224577 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Ig-like domain-containing protein Chain: C
Molecule details ›
Chain: C
Length: 277 amino acids
Theoretical weight: 32.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A140T913 (Residues: 25-300; Coverage: 80%)
Gene name: HLA-A
Sequence domains:
Beta-2-microglobulin Chain: D
Molecule details ›
Chain: D
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Melanoma-associated antigen 10 Chain: E
Molecule details ›
Chain: E
Length: 9 amino acids
Theoretical weight: 1.04 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P43363 (Residues: 254-262; Coverage: 2%)
Gene names: MAGE10, MAGEA10
T-cell receptor alpha chain Chain: A
Molecule details ›
Chain: A
Length: 206 amino acids
Theoretical weight: 22.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
T-cell receptor beta chain Chain: B
Molecule details ›
Chain: B
Length: 241 amino acids
Theoretical weight: 27.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 54.197Å b: 77.446Å c: 116.682Å
α: 90° β: 102.7° γ: 90°
R-values:
R R work R free
0.204 0.202 0.236
Expression systems:
  • Escherichia coli
  • Not provided

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