› 7qsu

X-ray diffraction
1.9Å resolution

Crystal structure of homing endonuclease-associated TliVMA intein (C1A, d333-339)

Released:
DOI: 10.2210/pdb7qsu/pdb
PDB entry 7qsu coloured by chain, front view.
PDB entry 7qsu coloured by chain, side view.
PDB entry 7qsu coloured by chain, top view.
Source organism: Thermococcus litoralis
Primary publication:
Structural Basis for the Propagation of Homing Endonuclease-Associated Inteins.
Beyer HM, Iwaï H
Front Mol Biosci 9 855511 (2022)
PMID: 35372505

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124789 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
V-type ATP synthase alpha chain Chain: A
Molecule details ›
Chain: A
Length: 374 amino acids
Theoretical weight: 42.27 KDa
Source organism: Thermococcus litoralis
Expression system: Escherichia coli
UniProt:
  • Canonical: H3ZKG9 (Residues: 235-666; Coverage: 37%)
Gene names: OCC_09254, atpA
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand EPE 1 x EPE
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 69.57Å b: 43.96Å c: 72.12Å
α: 90° β: 104.62° γ: 90°
R-values:
R R work R free
0.178 0.176 0.21
Expression system: Escherichia coli

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Citations

1 review citation

Genome Editing for Sustainable Crop Improvement and Mitigation of Biotic and Abiotic Stresses.
Hamdan et al. (2022)