› 7qut

X-ray diffraction
2.24Å resolution

serial synchrotron crystallographic structure of Drosophila Melanogaster (6-4) photolyase

Released:
DOI: 10.2210/pdb7qut/pdb
PDB entry 7qut coloured by chain, front view.
PDB entry 7qut coloured by chain, side view.
PDB entry 7qut coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structural basis of the radical pair state in photolyases and cryptochromes.
Cellini A, Shankar MK, Wahlgren WY, Nimmrich A, Furrer A, James D, Wranik M, Aumonier S, Beale EV, Dworkowski F, Standfuss J, Weinert T, Westenhoff S
Chem Commun (Camb) 58 4889-4892 (2022)
PMID: 35352724

Function and Biology Details

Reaction catalysed: 
Cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170883 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Photolyase/cryptochrome alpha/beta domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 508 amino acids
Theoretical weight: 58.85 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0E8P0 (Residues: 5-145, 147-201, 202-332, 333-337, 338-504; Coverage: 92%)
Gene names: 38D.25, 64-PL, 64PHR, CG2488, D6-4, Dm 64, Dmel\CG2488, Dmel_CG2488, phr(6-4), phr6-4
Sequence domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P41
Unit cell:
a: 103.6Å b: 103.6Å c: 52.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.15 0.191
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Insights into Molecular Structure of Pterins Suitable for Biomedical Applications.
Buglak et al. (2022)
2 other articles

1 mention without citation

Structural basis of the radical pair state in photolyases and cryptochromes.
Cellini et al. (2022)