› 7qv3

Electron Microscopy
5.14Å resolution

Bacillus subtilis MutS2-collided disome complex (MutS2 conf.2; Leading 70S)

Released:
DOI: 10.2210/pdb7qv3/pdb
PDB entry 7qv3 coloured by chain, front view.
PDB entry 7qv3 coloured by chain, side view.
PDB entry 7qv3 coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Bacterial ribosome collision sensing by a MutS DNA repair ATPase paralogue.
Cerullo F, Filbeck S, Patil PR, Hung HC, Xu H, Vornberger J, Hofer FW, Schmitt J, Kramer G, Bukau B, Hofmann K, Pfeffer S, Joazeiro CAP
Nature 603 509-514 (2022)
PMID: 35264791
Related structures: EMD-14159

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 55-mer (preferred)
PDBe Complex ID:
PDB-CPX-289389 (preferred)
Entry contents:
49 distinct polypeptide molecules
5 distinct RNA molecules
Macromolecules (54 distinct):
Large ribosomal subunit protein bL32 Chain: 0
Molecule details ›
Chain: 0
Length: 59 amino acids
Theoretical weight: 6.75 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
UniProt:
  • Canonical: O34687 (Residues: 1-59; Coverage: 100%)
Gene names: BSU15080, rpmF
Sequence domains: Ribosomal L32p protein family
Large ribosomal subunit protein bL33A Chain: 1
Molecule details ›
Chain: 1
Length: 49 amino acids
Theoretical weight: 5.92 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
UniProt:
  • Canonical: P56849 (Residues: 1-49; Coverage: 100%)
Gene names: BSU24900, rpmG1, rpmGA
Sequence domains: Ribosomal protein L33
Large ribosomal subunit protein bL34 Chain: 2
Large ribosomal subunit protein bL35 Chain: 3
Large ribosomal subunit protein bL36 Chain: 4
Large ribosomal subunit protein bL31 Chain: 6
Large ribosomal subunit protein uL2 Chain: C
Large ribosomal subunit protein uL3 Chain: D
Large ribosomal subunit protein uL4 Chain: E
Large ribosomal subunit protein uL5 Chain: F
Large ribosomal subunit protein uL6 Chain: G
Nascent chain Chain: I
Large ribosomal subunit protein uL13 Chain: J
Large ribosomal subunit protein uL14 Chain: K
Large ribosomal subunit protein uL15 Chain: L
Large ribosomal subunit protein uL16 Chain: M
Large ribosomal subunit protein bL17 Chain: N
Large ribosomal subunit protein uL18 Chain: O
Large ribosomal subunit protein bL19 Chain: P
Large ribosomal subunit protein bL20 Chain: Q
Large ribosomal subunit protein bL21 Chain: R
Large ribosomal subunit protein uL22 Chain: S
Large ribosomal subunit protein uL23 Chain: T
Large ribosomal subunit protein uL24 Chain: U
Large ribosomal subunit protein bL27 Chain: W
Large ribosomal subunit protein bL9 Chain: X
Large ribosomal subunit protein uL29 Chain: Y
Large ribosomal subunit protein uL30 Chain: Z
Small ribosomal subunit protein bS21 Chain: b
Small ribosomal subunit protein uS3 Chain: c
Small ribosomal subunit protein uS4 Chain: d
Small ribosomal subunit protein uS5 Chain: e
Small ribosomal subunit protein bS6 Chain: f
Small ribosomal subunit protein uS7 Chain: g
Small ribosomal subunit protein uS8 Chain: h
Small ribosomal subunit protein uS9 Chain: i
Small ribosomal subunit protein uS10 Chain: j
Small ribosomal subunit protein uS11 Chain: k
Small ribosomal subunit protein uS12 Chain: l
Small ribosomal subunit protein uS13 Chain: m
Small ribosomal subunit protein uS14B Chain: n
Small ribosomal subunit protein uS15 Chain: o
Small ribosomal subunit protein bS16 Chain: p
Small ribosomal subunit protein uS17 Chain: q
Small ribosomal subunit protein bS18 Chain: r
Small ribosomal subunit protein uS19 Chain: s
Small ribosomal subunit protein bS20 Chain: t
Large ribosomal subunit protein bL28 Chain: u
Putative endonuclease MutS2 Chains: v, w
Messenger RNA Chain: A
5S ribosomal RNA Chain: B
P-site tRNA Chain: H
23S ribosomal RNA Chain: V
16S ribosomal RNA Chain: a

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 5.14Å
Relevant EMDB volumes: EMD-14159

Quick links

Citations

1 review citation

Ribosome-associated quality-control mechanisms from bacteria to humans.
Filbeck et al. (2022)
15 other articles

1 mention without citation

Bacterial ribosome collision sensing by a MutS DNA repair ATPase paralogue.
Cerullo et al. (2022)