› 7qxq

X-ray diffraction
2.25Å resolution

Coelenteramide-bound Renilla-type luciferase (AncFT)

Released:
DOI: 10.2210/pdb7qxq/pdb
PDB entry 7qxq coloured by chain, front view.
PDB entry 7qxq coloured by chain, side view.
PDB entry 7qxq coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Catalytic mechanism for Renilla-type luciferases
Schenkmayerova A, Toul M, Pluskal D, Baatallah R, Gagnot G, Pinto GP, Santana VT, Stuchla M, Neugebauer P, Chaiyen P, Damborsky J, Bednar D, Janin YL, Prokop Z, Marek M
Nat Catal (2023)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-168709 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fragment transplantation onto hyperstable ancestor of haloalkane dehalogenases and Renilla luciferase (Anc-FT) Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 312 amino acids
Theoretical weight: 35.99 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand CEI 3 x CEI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 49.224Å b: 83.332Å c: 102.017Å
α: 90° β: 91.89° γ: 90°
R-values:
R R work R free
0.175 0.172 0.241
Expression system: Escherichia coli

Quick links