› 7r0x

X-ray diffraction
2.83Å resolution

Structure of the branching thioesterase from oocydin biosynthesis

Released:
DOI: 10.2210/pdb7r0x/pdb
PDB entry 7r0x coloured by chain, front view.
PDB entry 7r0x coloured by chain, side view.
PDB entry 7r0x coloured by chain, top view.
Source organism: Serratia
Primary publication:
Structure of a promiscuous thioesterase domain responsible for branching acylation in polyketide biosynthesis.
Fraley AE, Dieterich C, Mabesoone M, Minas HA, Meoded RA, Hemmerling F, Piel J
Angew Chem Int Ed Engl (2022)
PMID: 35903999

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104617 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyketide synthase Chain: A
Molecule details ›
Chain: A
Length: 364 amino acids
Theoretical weight: 41.1 KDa
Source organism: Serratia
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A318P693 (Residues: 3745-4108; Coverage: 7%)
Gene name: CT690_01900
Sequence domains: Thioesterase domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: I4
Unit cell:
a: 106.818Å b: 106.818Å c: 78.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.224 0.28
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Biosynthesis of Antifungal Solanimycin May Involve an Iterative Nonribosomal Peptide Synthetase Module.
Murphy et al. (2023)