› 7r4l

X-ray diffraction
2.6Å resolution

Crystal structure of human mitochondrial NAD kinase

Released:
DOI: 10.2210/pdb7r4l/pdb
PDB entry 7r4l coloured by chain, front view.
PDB entry 7r4l coloured by chain, side view.
PDB entry 7r4l coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human NADK2 reveals a dimeric organization and active site occlusion by lysine acetylation.
Mary C, Soflaee MH, Kesavan R, Gelin M, Brown H, Zacharias G, Mathews TP, Lemoff A, Lionne C, Labesse G, Hoxhaj G
Mol Cell (2022)
PMID: 35868311

Function and Biology Details

Reaction catalysed: 
ATP + NAD(+) = ADP + NADP(+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175530 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD kinase 2, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 391 amino acids
Theoretical weight: 44.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4G0N4 (Residues: 61-442; Coverage: 86%)
Gene names: C5orf33, MNADK, NADK2, NADKD1
Sequence domains: ATP-NAD kinase N-terminal domain

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
1 bound ligand:
Ligand FE 1 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P43212
Unit cell:
a: 68.406Å b: 68.406Å c: 223.224Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.178 0.235
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

NADPH and Mitochondrial Quality Control as Targets for a Circadian-Based Fasting and Exercise Therapy for the Treatment of Parkinson's Disease.
Curtis et al. (2022)
1 more

1 mention without citation

Crystal structure of human NADK2 reveals a dimeric organization and active site occlusion by lysine acetylation.
Mary et al. (2022)