› 7r8b

Electron Microscopy
3.1Å resolution

The structure of human ABCG5/ABCG8 supplemented with cholesterol

Released:
DOI: 10.2210/pdb7r8b/pdb
PDB entry 7r8b coloured by chain, front view.
PDB entry 7r8b coloured by chain, side view.
PDB entry 7r8b coloured by chain, top view.
Source organisms:
Primary publication:
Molecular basis of cholesterol efflux via ABCG subfamily transporters.
Sun Y, Wang J, Long T, Qi X, Donnelly L, Elghobashi-Meinhardt N, Esparza L, Cohen JC, Xie XS, Hobbs HH, Li X
OpenAccess logo Proc Natl Acad Sci U S A 118 (2021)
PMID: 34404721
Related structures: EMD-24314

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-212728 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
ATP-binding cassette sub-family G member 5 Chain: A
Molecule details ›
Chain: A
Length: 666 amino acids
Theoretical weight: 74.44 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q9H222 (Residues: 1-651; Coverage: 100%)
Gene name: ABCG5
Sequence domains:
ATP-binding cassette sub-family G member 8 Chain: B
Molecule details ›
Chain: B
Length: 715 amino acids
Theoretical weight: 80.37 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q9H221 (Residues: 1-673; Coverage: 100%)
Gene name: ABCG8
Sequence domains:
2C7 Fab heavy chain Chain: C
2C7 Fab light chain Chain: D

Ligands and Environments

1 bound ligand:
Ligand CLR 2 x CLR
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.1Å
Relevant EMDB volumes: EMD-24314
Expression systems:
  • Komagataella pastoris
  • Mus musculus

Quick links

Citations

12 review citations

Migrasomes, a new mode of intercellular communication.
Jiang et al. (2023)
11 more

4 mentions without citation

Snapshots of ABCG1 and ABCG5/G8: A Sterol's Journey to Cross the Cellular Membranes.
Rezaei et al. (2022)
3 more