Function and Biology Details
Reactions catalysed:
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
GTP + a 5'-diphospho-[mRNA] = diphosphate + a 5'-(5'-triphosphoguanosine)-[mRNA]
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- Ubiquitin-like domain
- Ubiquitin domain
- Ubiquitin-like domain superfamily
- Peptidase C16, coronavirus
- Ubiquilin
- Papain-like viral protease, palm and finger domains, coronavirus
- Papain-like protease, thumb domain superfamily, coronavirus
- NSP3, second ubiquitin-like (Ubl) domain, coronavirus
1 more domain
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138566 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Papain-like protease nsp3
Molecule details ›
Chains: A, C, E, G, I
Length: 318 amino acids
Theoretical weight: 35.93 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Coronavirus papain-like peptidase
Length: 318 amino acids
Theoretical weight: 35.93 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P0DTD1 (Residues: 1564-1878; Coverage: 4%)
Sequence domains: Coronavirus papain-like peptidase
Ubiquitin-like protein ISG15
Molecule details ›
Chains: B, D, F, H, J
Length: 159 amino acids
Theoretical weight: 17.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Ubiquitin family
Length: 159 amino acids
Theoretical weight: 17.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P05161 (Residues: 2-157; Coverage: 95%)
Sequence domains: Ubiquitin family
