7rhq

Electron Microscopy
3.53Å resolution

Cryo-EM structure of Xenopus Patched-1 in complex with GAS1 and Sonic Hedgehog

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-157112 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
SSD domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 1205 amino acids
Theoretical weight: 134.62 KDa
Source organism: Xenopus laevis
Expression system: Insecta environmental sample
UniProt:
  • Canonical: Q98SW6 (Residues: 1-1178; Coverage: 83%)
Gene names: Ptc-1, Ptc1, X-ptc1, XPtc1, patched, patched1, ptc1, ptch1, ptch1.S
Sequence domains:
Sonic hedgehog protein N-product Chain: C
Molecule details ›
Chain: C
Length: 192 amino acids
Theoretical weight: 21.38 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q15465 (Residues: 24-130, 131-197; Coverage: 40%)
Gene name: SHH
Sequence domains: Hedgehog amino-terminal signalling domain
Growth arrest-specific protein 1 Chain: G
Molecule details ›
Chain: G
Length: 282 amino acids
Theoretical weight: 29.55 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P54826 (Residues: 40-317; Coverage: 91%)
Gene name: GAS1
Sequence domains: GDNF/GAS1 domain

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.53Å
Relevant EMDB volumes: EMD-24466
Expression systems:
  • Insecta environmental sample
  • Homo sapiens