› 7ri7

Electron Microscopy
8Å resolution

The structure of BAM in MSP1D1 nanodiscs

Released:
DOI: 10.2210/pdb7ri7/pdb
PDB entry 7ri7 coloured by chain, front view.
PDB entry 7ri7 coloured by chain, side view.
PDB entry 7ri7 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Plasticity within the barrel domain of BamA mediates a hybrid-barrel mechanism by BAM.
Wu R, Bakelar JW, Lundquist K, Zhang Z, Kuo KM, Ryoo D, Pang YT, Sun C, White T, Klose T, Jiang W, Gumbart JC, Noinaj N
Nat Commun 12 7131 (2021)
PMID: 34880256
Related structures: EMD-24476

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-141767 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Outer membrane protein assembly factor BamA Chain: A
Molecule details ›
Chain: A
Length: 810 amino acids
Theoretical weight: 90.64 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A940 (Residues: 1-810; Coverage: 100%)
Gene names: JW0172, b0177, bamA, yaeT, yzzN, yzzY
Sequence domains:
Outer membrane protein assembly factor BamB Chain: B
Molecule details ›
Chain: B
Length: 392 amino acids
Theoretical weight: 41.92 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P77774 (Residues: 1-392; Coverage: 100%)
Gene names: JW2496, b2512, bamB, yfgL
Sequence domains:
Outer membrane protein assembly factor BamC Chain: C
Outer membrane protein assembly factor BamD Chain: D
Outer membrane protein assembly factor BamE Chain: E

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 8Å
Relevant EMDB volumes: EMD-24476
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Surveying membrane landscapes: a new look at the bacterial cell surface.
Lithgow et al. (2023)
4 more