› 7rja

Electron Microscopy
3Å resolution

Complex III2 from Candida albicans, inhibitor free

Released:
DOI: 10.2210/pdb7rja/pdb
PDB entry 7rja coloured by chain, front view.
PDB entry 7rja coloured by chain, side view.
PDB entry 7rja coloured by chain, top view.
Source organism: Candida albicans SC5314
Primary publication:
Rieske head domain dynamics and indazole-derivative inhibition of Candida albicans complex III.
Di Trani JM, Liu Z, Whitesell L, Brzezinski P, Cowen LE, Rubinstein JL
Structure 30 129-138.e4 (2022)
PMID: 34525326
Related structures: EMD-24482

Function and Biology Details

Reaction catalysed: 
Quinol + 2 ferricytochrome c(Side 2) = quinone + 2 ferrocytochrome c(Side 2) + 2 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octadecamer (preferred)
PDBe Complex ID:
PDB-CPX-103398 (preferred)
Entry contents:
9 distinct polypeptide molecules
Macromolecules (9 distinct):
Cytochrome b-c1 complex reductase subunit, mitochondrial Chains: A, J
Molecule details ›
Chains: A, J
Length: 439 amino acids
Theoretical weight: 48 KDa
Source organism: Candida albicans SC5314
UniProt:
  • Canonical: A0A1D8PP59 (Residues: 1-439; Coverage: 100%)
Gene names: CAALFM_C505230CA, COR1, orf19.11499
Sequence domains:
Cytochrome b Chains: K, T
Molecule details ›
Chains: K, T
Length: 387 amino acids
Theoretical weight: 43.74 KDa
Source organism: Candida albicans SC5314
UniProt:
  • Canonical: P0C8L0 (Residues: 1-387; Coverage: 100%)
Gene names: CM_00330W, COB, CYTB, CaalfMp11
Sequence domains:
Cytochrome b-c1 complex catalytic subunit, mitochondrial Chains: D, N
Molecule details ›
Chains: D, N
Length: 288 amino acids
Theoretical weight: 32.26 KDa
Source organism: Candida albicans SC5314
UniProt:
  • Canonical: A0A1D8PHA3 (Residues: 1-288; Coverage: 100%)
Gene names: CAALFM_C204950CA, CYT1, CYT12, orf19.11011
Sequence domains: Cytochrome C1 family
Cytochrome b-c1 complex subunit 7, mitochondrial Chains: G, Q
Molecule details ›
Chains: G, Q
Length: 127 amino acids
Theoretical weight: 14.44 KDa
Source organism: Candida albicans SC5314
UniProt:
  • Canonical: Q5ABS1 (Residues: 1-127; Coverage: 100%)
Gene names: CAALFM_C603400CA, QCR7, orf19.13074
Sequence domains: Ubiquinol-cytochrome C reductase complex 14kD subunit
Cytochrome b-c1 complex subunit 8, mitochondrial Chains: F, P
Molecule details ›
Chains: F, P
Length: 95 amino acids
Theoretical weight: 11.13 KDa
Source organism: Candida albicans SC5314
UniProt:
  • Canonical: A0A1D8PHA2 (Residues: 1-95; Coverage: 100%)
Gene names: CAALFM_C204590CA, QCR8, orf19.4490.2
Sequence domains: UcrQ family
Cytochrome b-c1 complex subunit 6, mitochondrial Chains: H, R
Molecule details ›
Chains: H, R
Length: 135 amino acids
Theoretical weight: 16.04 KDa
Source organism: Candida albicans SC5314
UniProt:
  • Canonical: A0A1D8PJT8 (Residues: 1-135; Coverage: 100%)
Gene names: CAALFM_C304080WA, QCR6, orf19.913.2
Sequence domains: Ubiquinol-cytochrome C reductase hinge protein
Cytochrome b-c1 complex subunit 9, mitochondrial Chains: I, S
Molecule details ›
Chains: I, S
Length: 65 amino acids
Theoretical weight: 7.52 KDa
Source organism: Candida albicans SC5314
UniProt:
  • Canonical: A0A1D8PLP3 (Residues: 1-65; Coverage: 100%)
Gene names: CAALFM_C402890CA, QCR9, orf19.2707.1
Sequence domains: Ubiquinol-cytochrome C reductase, UQCRX/QCR9 like
Cytochrome b-c1 complex subunit Rieske, mitochondrial Chains: C, M
Molecule details ›
Chains: C, M
Length: 213 amino acids
Theoretical weight: 23.23 KDa
Source organism: Candida albicans SC5314
UniProt:
  • Canonical: A0A1D8PJX3 (Residues: 1-213; Coverage: 100%)
Gene names: CAALFM_C304430WA, RIP1, orf19.13314
Sequence domains:
Cytochrome b-c1 complex subunit 2, mitochondrial Chains: B, L
Molecule details ›
Chains: B, L
Length: 374 amino acids
Theoretical weight: 39.59 KDa
Source organism: Candida albicans SC5314
UniProt:
  • Canonical: P83782 (Residues: 1-374; Coverage: 100%)
Gene names: CAALFM_C503350WA, CaO19.10167, CaO19.2644, QCR2
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand HEM 4 x HEM
Ligand U10 4 x U10
Ligand HEC 2 x HEC
Ligand FES 2 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-24482

Quick links

Citations

7 citation in other articles

Cryo-EM structure of the respiratory I + III2 supercomplex from Arabidopsis thaliana at 2 Å resolution.
Klusch et al. (2023)
6 more

2 mentions without citation

Quinone binding sites of cyt bc complexes analysed by X-ray crystallography and cryogenic electron microscopy.
Kao et al. (2022)
1 more