› 7rk0

X-ray diffraction
2.28Å resolution

Crystal structure of Thermovibrio ammonificans THI4

Released:
DOI: 10.2210/pdb7rk0/pdb
PDB entry 7rk0 coloured by chain, front view.
PDB entry 7rk0 coloured by chain, side view.
PDB entry 7rk0 coloured by chain, top view.
Source organism: Thermovibrio ammonificans
Primary publication:
Structure and function of aerotolerant, multiple-turnover THI4 thiazole synthases.
Joshi J, Li Q, García-García JD, Leong BJ, Hu Y, Bruner SD, Hanson AD
Biochem J 478 3265-3279 (2021)
PMID: 34409984

Function and Biology Details

Reaction catalysed: 
NAD(+) + glycine + sulfide = nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123124 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiamine thiazole synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 268 amino acids
Theoretical weight: 28.77 KDa
Source organism: Thermovibrio ammonificans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: E8T346 (Residues: 1-268; Coverage: 100%)
Gene names: Theam_0077, thi4
Sequence domains: Thi4 family

Ligands and Environments

2 bound ligands:
Ligand FE 4 x FE
Ligand 48H 4 x 48H
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Unit cell:
a: 89.889Å b: 89.685Å c: 131.813Å
α: 90° β: 96.979° γ: 90°
R-values:
R R work R free
0.221 0.218 0.277
Expression system: Escherichia coli BL21(DE3)

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Citations

2 citation in other articles

Using continuous directed evolution to improve enzymes for plant applications.
García-García et al. (2022)
1 more

2 mentions without citation

Structure and function of aerotolerant, multiple-turnover THI4 thiazole synthases.
Joshi et al. (2021)
1 more