› 7rk7

X-ray diffraction
2.54Å resolution

The complex between TIL 1383i TCR and human Class I MHC HLA-A2 with the bound Tyrosinase(369-377)(N371D) nonameric peptide

Released:
DOI: 10.2210/pdb7rk7/pdb
PDB entry 7rk7 coloured by chain, front view.
PDB entry 7rk7 coloured by chain, side view.
PDB entry 7rk7 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Singh NK, Davancaze LM, Arbuiso A, Weiss LI, Keller GLJ, Baker BM

Function and Biology Details

Reaction catalysed: 
(1a) L-tyrosine + 1/2 O(2) = L-dopa
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-225029 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A alpha chain Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 31.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Tyrosinase Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.03 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P14679 (Residues: 369-377; Coverage: 2%)
Gene name: TYR
TIL1383i (h3T) T cell receptor alpha chain Chain: D
Molecule details ›
Chain: D
Length: 214 amino acids
Theoretical weight: 23.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
TIL1383i (h3T) T cell receptor beta chain Chain: E
Molecule details ›
Chain: E
Length: 253 amino acids
Theoretical weight: 28.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2
Unit cell:
a: 114.738Å b: 74.428Å c: 131.058Å
α: 90° β: 93.487° γ: 90°
R-values:
R R work R free
0.214 0.211 0.255
Expression systems:
  • Escherichia coli
  • Not provided
  • Escherichia coli BL21(DE3)

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