› 7rmw

X-ray diffraction
2.45Å resolution

Crystal structure of B. subtilis PurR bound to ppGpp

Released:
DOI: 10.2210/pdb7rmw/pdb
PDB entry 7rmw coloured by chain, front view.
PDB entry 7rmw coloured by chain, side view.
PDB entry 7rmw coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
The nucleotide messenger (p)ppGpp is an anti-inducer of the purine synthesis transcription regulator PurR in Bacillus.
Anderson BW, Schumacher MA, Yang J, Turdiev A, Turdiev H, Schroeder JW, He Q, Lee VT, Brennan RG, Wang JD
Nucleic Acids Res 50 847-866 (2022)
PMID: 34967415

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153426 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pur operon repressor Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 285 amino acids
Theoretical weight: 31.26 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P37551 (Residues: 1-285; Coverage: 100%)
Gene names: BSU00470, purR, yabI
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand G4P 4 x G4P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P1
Unit cell:
a: 60.264Å b: 90.969Å c: 98.708Å
α: 62.75° β: 75.36° γ: 78.53°
R-values:
R R work R free
0.196 0.194 0.24
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Diverse molecular mechanisms of transcription regulation by the bacterial alarmone ppGpp.
Travis et al. (2022)
5 more

1 mention without citation

The nucleotide messenger (p)ppGpp is an anti-inducer of the purine synthesis transcription regulator PurR in Bacillus.
Anderson et al. (2022)