› 7rom

X-ray diffraction
1.65Å resolution

Crystal structure of Saccharomyces cerevisiae NADH-cytochrome b5 reductase 1 (Cbr1) fragment (residues 28-284) bound to FAD

Released:
DOI: 10.2210/pdb7rom/pdb
PDB entry 7rom coloured by chain, front view.
PDB entry 7rom coloured by chain, side view.
PDB entry 7rom coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Entry authors: Fenwick MK, Zhang Y, Lin H

Function and Biology Details

Reaction catalysed: 
NADH + 2 ferricytochrome b5 = NAD(+) + H(+) + 2 ferrocytochrome b5
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153685 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADH-cytochrome b5 reductase 1 Chain: A
Molecule details ›
Chain: A
Length: 261 amino acids
Theoretical weight: 29.03 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38626 (Residues: 28-284; Coverage: 91%)
Gene names: CBR, CBR1, CBR5, YIL043C
Sequence domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
2 bound ligands:
Ligand CL 3 x CL
Ligand PGE 2 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P43212
Unit cell:
a: 72.225Å b: 72.225Å c: 114.669Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.155 0.188
Expression system: Escherichia coli BL21(DE3)

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