› 7rpk

Electron Microscopy
2.7Å resolution

Cryo-EM structure of murine Dispatched in complex with Sonic hedgehog

Released:
DOI: 10.2210/pdb7rpk/pdb
PDB entry 7rpk coloured by chain, front view.
PDB entry 7rpk coloured by chain, side view.
PDB entry 7rpk coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Dispatched uses Na+ flux to power release of lipid-modified Hedgehog.
Wang Q, Asarnow DE, Ding K, Mann RK, Hatakeyama J, Zhang Y, Ma Y, Cheng Y, Beachy PA
Nature 599 320-324 (2021)
PMID: 34707294
Related structures: EMD-24617

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174814 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein dispatched homolog 1 Chain: A
Molecule details ›
Chain: A
Length: 1352 amino acids
Theoretical weight: 152.07 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q3TDN0 (Residues: 172-1521; Coverage: 89%)
Gene names: Disp, Disp1, Dispa, Icb, Icbins
Sequence domains: Patched family
Sonic hedgehog protein N-product Chain: H
Molecule details ›
Chain: H
Length: 164 amino acids
Theoretical weight: 18.73 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q62226 (Residues: 26-189; Coverage: 40%)
Gene names: Hhg1, Shh
Sequence domains: Hedgehog amino-terminal signalling domain

Ligands and Environments

8 bound ligands:
Ligand Y01 26 x Y01
Ligand AV0 2 x AV0
Ligand NA 2 x NA
Ligand 6OE 1 x 6OE
Ligand NAG 5 x NAG
Ligand CA 2 x CA
Ligand ZN 1 x ZN
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.7Å
Relevant EMDB volumes: EMD-24617
Expression systems:
  • Homo sapiens
  • Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

The Intimate Connection Between Lipids and Hedgehog Signaling.
Nguyen et al. (2022)
3 more