› 7rwy

X-ray diffraction
2.2Å resolution

Crystal structure of a Fe-bound RIDC1 variant in the presence of reductant

Released:
DOI: 10.2210/pdb7rwy/pdb
PDB entry 7rwy coloured by chain, front view.
PDB entry 7rwy coloured by chain, side view.
PDB entry 7rwy coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Redox- and metal-directed structural diversification in designed metalloprotein assemblies.
Kakkis A, Golub E, Choi TS, Tezcan FA
Chem Commun (Camb) 58 6958-6961 (2022)
PMID: 35642584

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142157 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble cytochrome b562 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 106 amino acids
Theoretical weight: 11.66 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene name: cybC
Sequence domains: Cytochrome b562

Ligands and Environments

4 bound ligands:
Ligand HEC 6 x HEC
Ligand 1PE 2 x 1PE
Ligand FE 4 x FE
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 52.55Å b: 82.68Å c: 74.14Å
α: 90° β: 95.22° γ: 90°
R-values:
R R work R free
0.205 0.202 0.259
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Atomically Accurate Design of Metalloproteins with Predefined Coordination Geometries.
Hoffnagle et al. (2023)

1 mention without citation

Redox- and metal-directed structural diversification in designed metalloprotein assemblies.
Kakkis et al. (2022)