› 7s0k

X-ray diffraction
2.3Å resolution

HAP2 from Cyanidioschyzon merolae

Released:
DOI: 10.2210/pdb7s0k/pdb
PDB entry 7s0k coloured by chain, front view.
PDB entry 7s0k coloured by chain, side view.
PDB entry 7s0k coloured by chain, top view.
Source organism: Cyanidioschyzon merolae
Primary publication:
Monomeric prefusion structure of an extremophile gamete fusogen and stepwise formation of the postfusion trimeric state.
Feng J, Dong X, Su Y, Lu C, Springer TA
Nat Commun 13 4064 (2022)
PMID: 35831325

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125962 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Generative cell specific-1/HAP2 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 545 amino acids
Theoretical weight: 59.43 KDa
Source organism: Cyanidioschyzon merolae
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: M1V8A6 (Residues: 28-564; Coverage: 76%)
Gene name: CYME_CMK076C
Sequence domains: Male gamete fusion factor

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
3 bound ligands:
Ligand NAG 1 x NAG
Ligand GOL 3 x GOL
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2221
Unit cell:
a: 102.96Å b: 197.01Å c: 74.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.223 0.264
Expression system: Drosophila melanogaster

Quick links

Citations

1 citations in other articles

A novel function for the sperm adhesion protein IZUMO1 in cell-cell fusion.
Brukman et al. (2023)

2 mentions without citation

A Frame-by-Frame Glance at Membrane Fusion Mechanisms: From Viral Infections to Fertilization.
Azimi et al. (2023)
1 more