› 7s0p

X-ray diffraction
1.3Å resolution

Crystal structure of Porcine Factor VIII C2 Domain Bound to Phosphatidylserine

Released:
DOI: 10.2210/pdb7s0p/pdb
PDB entry 7s0p coloured by chain, front view.
PDB entry 7s0p coloured by chain, side view.
PDB entry 7s0p coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Stable binding to phosphatidylserine-containing membranes requires conserved arginine residues in tandem C domains of blood coagulation factor VIII.
Peters SC, Childers KC, Mitchell CE, Avery NG, Reese SS, Mitchell C, Wo SW, Swanson CD, Brison CM, Spiegel PC
Front Mol Biosci 9 1040106 (2022)
PMID: 36387287

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146106 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Coagulation factor VIII Chain: M
Molecule details ›
Chain: M
Length: 157 amino acids
Theoretical weight: 17.5 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli B
UniProt:
  • Canonical: P12263 (Residues: 1974-2129; Coverage: 7%)
Gene names: CF8, F8
Sequence domains: F5/8 type C domain

Ligands and Environments

1 bound ligand:
Ligand SEP 1 x SEP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: I222
Unit cell:
a: 49.072Å b: 68.323Å c: 105.987Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.137 0.136 0.156
Expression system: Escherichia coli B

Quick links

Citations

1 mention without citation

Stable binding to phosphatidylserine-containing membranes requires conserved arginine residues in tandem C domains of blood coagulation factor VIII.
Peters et al. (2022)