› 7s6d

Electron Microscopy
3.1Å resolution

CryoEM structure of modular PKS holo-Lsd14 bound to antibody fragment 1B2, composite structure

Released:
DOI: 10.2210/pdb7s6d/pdb
PDB entry 7s6d coloured by chain, front view.
PDB entry 7s6d coloured by chain, side view.
PDB entry 7s6d coloured by chain, top view.
Source organisms:
Primary publication:
Modular polyketide synthase contains two reaction chambers that operate asynchronously.
Bagde SR, Mathews II, Fromme JC, Kim CY
Science 374 723-729 (2021)
PMID: 34735234
Related structures: EMD-24875

Function and Biology Details

Reaction catalysed: 
Propanoyl-CoA + 6 (2S)-methylmalonyl-CoA + 6 NADPH = 6-deoxyerythronolide B + 7 CoA + 6 CO(2) + H(2)O + 6 NADP(+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-207682 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Erythronolide synthase EryA2; Putative polyketide synthase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1649 amino acids
Theoretical weight: 174.09 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: B6ZK67 (Residues: 4-4, 38-1647; Coverage: 98%)
  • Canonical: Q03132 (Residues: 2-30; Coverage: 1%)
Gene names: eryA, lsd14
Sequence domains:
Fab 1B2 heavy chain Chains: D, E
Molecule details ›
Chains: D, E
Length: 249 amino acids
Theoretical weight: 26.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Fab 1B2 light chain Chains: F, G

Ligands and Environments

1 bound ligand:
Ligand ATR 1 x ATR
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.1Å
Relevant EMDB volumes: EMD-24875
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Enzymology of assembly line synthesis by modular polyketide synthases.
Grininger M. (2023)
4 more

1 mention without citation

Sea Urchin Polyketide Synthase SpPks1 Produces the Naphthalene Precursor to Echinoderm Pigments.
Li et al. (2022)