› 7sci

X-ray diffraction
1.9Å resolution

AM0627 metallopeptidase from Akkermansia muciniphila

Released:
DOI: 10.2210/pdb7sci/pdb
PDB entry 7sci coloured by chain, front view.
PDB entry 7sci coloured by chain, side view.
PDB entry 7sci coloured by chain, top view.
Source organism: Akkermansia muciniphila ATCC BAA-835
Primary publication:
Structure-guided mutagenesis of a mucin-selective metalloprotease from Akkermansia muciniphila alters substrate preferences.
Shon DJ, Fernandez D, Riley NM, Ferracane MJ, Bertozzi CR
J Biol Chem 298 101917 (2022)
PMID: 35405095

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109737 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidase M60 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 487 amino acids
Theoretical weight: 55.31 KDa
Source organism: Akkermansia muciniphila ATCC BAA-835
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B2UPI7 (Residues: 21-506; Coverage: 100%)
Gene name: Amuc_0627
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 1 x ZN
Ligand PGE 1 x PGE
Ligand FMT 8 x FMT
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P65
Unit cell:
a: 101.564Å b: 101.564Å c: 114.626Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.171 0.17 0.202
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Measuring the multifaceted roles of mucin-domain glycoproteins in cancer.
Riley et al. (2023)
1 more

4 mentions without citation

Molecular Recognition of GalNAc in Mucin-Type O-Glycosylation.
Sanz-Martinez et al. (2023)
3 more