› 7sf9

X-ray diffraction
1.8Å resolution

Branchiostoma Floridae Violet Fluorescent Protein

Released:
DOI: 10.2210/pdb7sf9/pdb
PDB entry 7sf9 coloured by chain, front view.
PDB entry 7sf9 coloured by chain, side view.
PDB entry 7sf9 coloured by chain, top view.
Source organism: Branchiostoma floridae
Primary publication:
A novel violet fluorescent protein contains a unique oxidized tyrosine as the simplest chromophore ever reported in fluorescent proteins.
Roldán-Salgado A, Muslinkina L, Pletnev S, Pletneva N, Pletnev V, Gaytán P
Protein Sci 31 688-700 (2022)
PMID: 34936154

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111271 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Violet Fluorescent Protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 238 amino acids
Theoretical weight: 26.92 KDa
Source organism: Branchiostoma floridae
Expression system: Escherichia coli
UniProt:
  • Canonical: C3YRA2 (Residues: 10-226; Coverage: 96%)
Gene name: BRAFLDRAFT_75522
Sequence domains: Green fluorescent protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P65
Unit cell:
a: 177.674Å b: 177.674Å c: 52.362Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.209 0.263
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Extension of the short wavelength side of fluorescent proteins using hydrated chromophores, and its application.
Sugiura et al. (2022)

1 mention without citation

A novel violet fluorescent protein contains a unique oxidized tyrosine as the simplest chromophore ever reported in fluorescent proteins.
Roldán-Salgado et al. (2022)