7shf

Electron Microscopy
3.4Å resolution

Cryo-EM structure of GPR158 coupled to the RGS7-Gbeta5 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-156050 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Regulator of G-protein signaling 7 Chain: C
Molecule details ›
Chain: C
Length: 469 amino acids
Theoretical weight: 54.76 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P49802 (Residues: 1-463; Coverage: 94%)
Gene name: RGS7
Sequence domains:
Guanine nucleotide-binding protein subunit beta-5 Chain: D
Molecule details ›
Chain: D
Length: 353 amino acids
Theoretical weight: 38.78 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: P62881 (Residues: 43-395; Coverage: 89%)
Gene name: Gnb5
Sequence domains: WD domain, G-beta repeat
Metabotropic glycine receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 781 amino acids
Theoretical weight: 88.25 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q5T848 (Residues: 1-775; Coverage: 63%)
Gene names: GPR158, KIAA1136
Sequence domains: 7 transmembrane sweet-taste receptor of 3 GCPR

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-25126
Expression system: Homo sapiens