› 7snm

X-ray diffraction
2.55Å resolution

Lanosterol-bound P450 domain of the CYP51-ferredoxin fusion protein from Methylococcus capsulatus

Released:
DOI: 10.2210/pdb7snm/pdb
Model geometry
Fit model/data
PDB entry 7snm coloured by chain, front view.
PDB entry 7snm coloured by chain, side view.
PDB entry 7snm coloured by chain, top view.
Source organism: Methylococcus capsulatus str. Bath
Primary publication:
Unravelling the role of transient redox partner complexes in P450 electron transfer mechanics.
Hargrove TY, Lamb DC, Smith JA, Wawrzak Z, Kelly SL, Lepesheva GI
OpenAccess logo Sci Rep 12 16232 (2022)
PMID: 36171457

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178784 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4Fe-4S ferredoxin-type domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 551 amino acids
Theoretical weight: 62.48 KDa
Source organism: Methylococcus capsulatus str. Bath
Expression system: Escherichia coli
UniProt:
  • Canonical: Q603T8 (Residues: 1-551; Coverage: 100%)
Gene names: MCA2711, cyp51
Sequence domains:

Ligands and Environments


Cofactor: Ligand HEM 4 x HEM
1 bound ligand:
Ligand LAN 4 x LAN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 73.21Å b: 206.16Å c: 81.43Å
α: 90° β: 114.09° γ: 90°
R-values:
R R work R free
0.207 0.204 0.246
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Structural insights into 3Fe-4S ferredoxins diversity in M. tuberculosis highlighted by a first redox complex with P450.
Gilep et al. (2022)

2 mentions without citation

Unravelling the role of transient redox partner complexes in P450 electron transfer mechanics.
Hargrove et al. (2022)
1 more