› 7snu

X-ray diffraction
1.46Å resolution

Crystal structure of ShHTL7 from Striga hermonthica in complex with strigolactone antagonist RG6

Released:
DOI: 10.2210/pdb7snu/pdb
PDB entry 7snu coloured by chain, front view.
PDB entry 7snu coloured by chain, side view.
PDB entry 7snu coloured by chain, top view.
Source organism: Striga hermonthica
Primary publication:
A novel strigolactone receptor antagonist provides insights into the structural inhibition, conditioning, and germination of the crop parasite Striga.
Arellano-Saab A, McErlean CSP, Lumba S, Savchenko A, Stogios PJ, McCourt P
J Biol Chem 298 101734 (2022)
PMID: 35181340

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102500 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 272 amino acids
Theoretical weight: 30.26 KDa
Source organism: Striga hermonthica
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0M3PNA2 (Residues: 1-271; Coverage: 100%)
Sequence domains: Alpha/beta hydrolase family

Ligands and Environments

5 bound ligands:
Ligand GOL 2 x GOL
Ligand ACT 5 x ACT
Ligand MG 1 x MG
Ligand IMD 1 x IMD
Ligand HFJ 1 x HFJ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P65
Unit cell:
a: 96.216Å b: 96.216Å c: 69.358Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.129 0.128 0.162
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Expansion of the Strigolactone Profluorescent Probes Repertory: The Right Probe for the Right Application.
de Saint Germain et al. (2022)
1 more

1 mention without citation

A novel strigolactone receptor antagonist provides insights into the structural inhibition, conditioning, and germination of the crop parasite Striga.
Arellano-Saab et al. (2022)