› 7spr

X-ray diffraction
1.79Å resolution

Crystal structure of SMG1 mutant (G28C/P206C/Q34P/A37P/M176V/G177A/M294R/F278N)

Released:
DOI: 10.2210/pdb7spr/pdb
PDB entry 7spr coloured by chain, front view.
PDB entry 7spr coloured by chain, side view.
PDB entry 7spr coloured by chain, top view.
Source organism: Malassezia globosa CBS 7966
Entry authors: Li LL, Wang YH

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108770 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Secreted mono- and diacylglycerol lipase LIP1 Chain: A
Molecule details ›
Chain: A
Length: 277 amino acids
Theoretical weight: 30.9 KDa
Source organism: Malassezia globosa CBS 7966
Expression system: Komagataella phaffii
UniProt:
  • Canonical: A8PUY1 (Residues: 28-304; Coverage: 97%)
Gene names: LIP1, MGL_0797, SMG1
Sequence domains: Lipase (class 3)

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P212121
Unit cell:
a: 48.053Å b: 48.996Å c: 110.804Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.157 0.183
Expression system: Komagataella phaffii

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