› 7su0

X-ray diffraction
2.41Å resolution

Crystal structure of an acidic pH-selective Ipilimumab variant Ipi.105 in complex with CTLA-4

Released:
DOI: 10.2210/pdb7su0/pdb
PDB entry 7su0 coloured by chain, front view.
PDB entry 7su0 coloured by chain, side view.
PDB entry 7su0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Improved therapeutic index of an acidic pH-selective antibody.
Lee PS, MacDonald KG, Massi E, Chew PV, Bee C, Perkins P, Chau B, Thudium K, Lohre J, Nandi P, Deyanova EG, Barman I, Gudmundsson O, Dollinger G, Sproul T, Engelhardt JJ, Strop P, Rajpal A
MAbs 14 2024642
PMID: 35192429

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210689 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cytotoxic T-lymphocyte protein 4 Chains: C, D
Molecule details ›
Chains: C, D
Length: 125 amino acids
Theoretical weight: 13.56 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P16410 (Residues: 36-153; Coverage: 63%)
Gene names: CD152, CTLA4
Sequence domains: Immunoglobulin V-set domain
Fab heavy chain Chains: H, I
Molecule details ›
Chains: H, I
Length: 229 amino acids
Theoretical weight: 24.75 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Fab light chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 215 amino acids
Theoretical weight: 23.53 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

3 bound ligands:
Ligand NAG 3 x NAG
Ligand FLC 1 x FLC
Ligand PEG 1 x PEG
1 modified residue:
Ligand PCA 2 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: C2221
Unit cell:
a: 96.73Å b: 197.84Å c: 146.49Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.264
Expression system: Homo sapiens

Quick links

Citations

4 review citations

Signaling pathways and targeted therapies in lung squamous cell carcinoma: mechanisms and clinical trials.
Niu et al. (2022)
3 more