› 7t0r

X-ray diffraction
3.65Å resolution

Crystal structure of the anti-CD4 adnectin 6940_B01 as a complex with the extracellular domains of CD4 and ibalizumab fAb

Released:
DOI: 10.2210/pdb7t0r/pdb
Model geometry
Fit model/data
PDB entry 7t0r coloured by chain, front view.
PDB entry 7t0r coloured by chain, side view.
PDB entry 7t0r coloured by chain, top view.
Source organisms:
Primary publication:
Novel Bent Conformation of CD4 Induced by HIV-1 Inhibitor Indirectly Prevents Productive Viral Attachment.
Wensel D, Williams S, Dixon DP, Ward P, McCormick P, Concha N, Stewart E, Hong X, Mazzucco C, Pal S, Ding B, Fellinger C, Krystal M
J Mol Biol 434 167395 (2022)
PMID: 34896364

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-279435 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Ibalizumab Light Chain Chains: B, L
Molecule details ›
Chains: B, L
Length: 219 amino acids
Theoretical weight: 24.25 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
Ibalizumab Heavy Chain Chains: C, H
Molecule details ›
Chains: C, H
Length: 229 amino acids
Theoretical weight: 24.79 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
T-cell surface glycoprotein CD4 Chains: A, D
Adnectin 6940_B01 Chains: G, I

Ligands and Environments

2 bound ligands:
Ligand NAG 2 x NAG
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 22-ID
Unit cell:
a: 83.51Å b: 164.014Å c: 167.053Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.226 0.292
Expression systems:
  • Cricetulus griseus
  • Homo sapiens
  • Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

DNA framework signal amplification platform-based high-throughput systemic immune monitoring.
Chen et al. (2024)