› 7t7z

X-ray diffraction
1.46Å resolution

The crystal structure of family 8 carbohydrate-binding module from Dictyostelium discoideum

Released:
DOI: 10.2210/pdb7t7z/pdb
Model geometry
Fit model/data
PDB entry 7t7z coloured by chain, front view.
PDB entry 7t7z coloured by chain, side view.
PDB entry 7t7z coloured by chain, top view.
Source organism: Dictyostelium discoideum
Primary publication:
Unique properties of a Dictyostelium discoideum carbohydrate-binding module expand our understanding of CBM-ligand interactions.
Liberato MV, Campos BM, Tomazetto G, Crouch LI, Garcia W, Zeri ACM, Bolam DN, Squina FM
OpenAccess logo J Biol Chem 298 101891 (2022)
PMID: 35378128

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149777 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase Chain: A
Molecule details ›
Chain: A
Length: 154 amino acids
Theoretical weight: 16.61 KDa
Source organism: Dictyostelium discoideum
Expression system: Escherichia coli
UniProt:
  • Canonical: P22699 (Residues: 555-705; Coverage: 22%)
Gene names: DDB_G0271134, celA

Ligands and Environments

3 bound ligands:
Ligand EDO 1 x EDO
Ligand NA 1 x NA
Ligand LMR 1 x LMR
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: LNLS BEAMLINE W01B-MX2
Unit cell:
a: 36.739Å b: 47.27Å c: 79.449Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.152 0.191
Expression system: Escherichia coli

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Citations

3 citation in other articles

Carbohydrate-binding modules enhance H2O2 tolerance by promoting lytic polysaccharide monooxygenase active site H2O2 consumption.
Gao et al. (2024)
2 more

1 mention without citation

Unique properties of a Dictyostelium discoideum carbohydrate-binding module expand our understanding of CBM-ligand interactions.
Liberato et al. (2022)