› 7thg

X-ray diffraction
2.9Å resolution

Crystal Structure Of Human NADH-Cytochrome B5 Reductase

Released:
DOI: 10.2210/pdb7thg/pdb
PDB entry 7thg coloured by chain, front view.
PDB entry 7thg coloured by chain, side view.
PDB entry 7thg coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Zheng A, Thibodeau PH

Function and Biology Details

Reaction catalysed: 
NADH + 2 ferricytochrome b5 = NAD(+) + H(+) + 2 ferrocytochrome b5
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132457 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADH-cytochrome b5 reductase 3 Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00387 (Residues: 28-301; Coverage: 91%)
Gene names: CYB5R3, DIA1
Sequence domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P41
Unit cell:
a: 64.83Å b: 64.83Å c: 71.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.243 0.274
Expression system: Escherichia coli

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