› 7thj

Electron Microscopy
3.8Å resolution

Structure of the yeast clamp loader (Replication Factor C RFC) bound to the sliding clamp (Proliferating Cell Nuclear Antigen PCNA) in an autoinhibited conformation

Released:
DOI: 10.2210/pdb7thj/pdb
PDB entry 7thj coloured by chain, front view.
PDB entry 7thj coloured by chain, side view.
PDB entry 7thj coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Cryo-EM structures reveal high-resolution mechanism of a DNA polymerase sliding clamp loader.
Gaubitz C, Liu X, Pajak J, Stone NP, Hayes JA, Demo G, Kelch BA
OpenAccess logo Elife 11 (2022)
PMID: 35179493
Related structures: EMD-25568

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147512 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Replication factor C subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 861 amino acids
Theoretical weight: 95.05 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38630 (Residues: 1-861; Coverage: 100%)
Gene names: CDC44, RFC1, YOR217W, YOR50-7
Sequence domains:
Replication factor C subunit 4 Chain: B
Molecule details ›
Chain: B
Length: 323 amino acids
Theoretical weight: 36.2 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40339 (Residues: 1-323; Coverage: 100%)
Gene names: O0923, RFC4, YOL094C
Sequence domains:
Replication factor C subunit 3 Chain: C
Replication factor C subunit 2 Chain: D
Replication factor C subunit 5 Chain: E
Proliferating cell nuclear antigen Chains: F, G, H

Ligands and Environments

3 bound ligands:
Ligand AGS 4 x AGS
Ligand MG 4 x MG
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.8Å
Relevant EMDB volumes: EMD-25568
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 citation in other articles

Multistep loading of a DNA sliding clamp onto DNA by replication factor C.
Schrecker et al. (2022)
14 more

1 mention without citation

Cryo-EM structures reveal high-resolution mechanism of a DNA polymerase sliding clamp loader.
Gaubitz et al. (2022)