› 7tlr

X-ray diffraction
2.1Å resolution

Structure of Atopobium parvulum SufS A34Y mutant

Released:
DOI: 10.2210/pdb7tlr/pdb
PDB entry 7tlr coloured by chain, front view.
PDB entry 7tlr coloured by chain, side view.
PDB entry 7tlr coloured by chain, top view.
Source organism: Lancefieldella parvula
Primary publication:
Structural analysis of Atopobium parvulum SufS cysteine desulfurase linked to Crohn's disease.
Karunakaran G, Yang Y, Tremblay V, Ning Z, Martin J, Belaouad A, Figeys D, Brunzelle JS, Giguere PM, Stintzi A, Couture JF
FEBS Lett 596 898-909 (2022)
PMID: 35122247

Function and Biology Details

Reaction catalysed: 
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111807 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine desulfurase Chains: A, B
Molecule details ›
Chains: A, B
Length: 447 amino acids
Theoretical weight: 49.28 KDa
Source organism: Lancefieldella parvula
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C8W9P2 (Residues: 1-429; Coverage: 100%)
Gene name: Apar_0399
Sequence domains: Aminotransferase class-V

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 76.314Å b: 86.78Å c: 133.438Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.211 0.251
Expression system: Escherichia coli BL21(DE3)

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Citations

2 citation in other articles

Using Nanopore Sequencing to Obtain Complete Bacterial Genomes from Saliva Samples.
Baker JL. (2022)
1 more