› 7tok

X-ray diffraction
2.45Å resolution

Crystal structure of the CBM domain of carbohydrate esterase FjoAcXE

Released:
DOI: 10.2210/pdb7tok/pdb
PDB entry 7tok coloured by chain, front view.
PDB entry 7tok coloured by chain, side view.
PDB entry 7tok coloured by chain, top view.
Source organism: Flavobacterium johnsoniae
Primary publication:
Elucidating Sequence and Structural Determinants of Carbohydrate Esterases for Complete Deacetylation of Substituted Xylans.
Penttinen L, Kouhi V, Fauré R, Skarina T, Stogios P, Master E, Jurak E
Molecules 27 (2022)
PMID: 35566004

Function and Biology Details

Reaction catalysed: 
Deacetylation of xylans and xylo-oligosaccharides
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105452 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SGNH hydrolase-type esterase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 150 amino acids
Theoretical weight: 16.39 KDa
Source organism: Flavobacterium johnsoniae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A5P6A8B9 (Residues: 58-207; Coverage: 38%)
Gene name: axeA

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2221
Unit cell:
a: 60.084Å b: 120.964Å c: 86.645Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.23 0.284
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

Elucidating Sequence and Structural Determinants of Carbohydrate Esterases for Complete Deacetylation of Substituted Xylans.
Penttinen et al. (2022)