› 7tr8

Electron Microscopy
3.6Å resolution

Cascade complex from type I-A CRISPR-Cas system

Released:
DOI: 10.2210/pdb7tr8/pdb
PDB entry 7tr8 coloured by chain, front view.
PDB entry 7tr8 coloured by chain, side view.
PDB entry 7tr8 coloured by chain, top view.
Source organism: Pyrococcus furiosus DSM 3638
Primary publication:
Allosteric control of type I-A CRISPR-Cas3 complexes and establishment as effective nucleic acid detection and human genome editing tools.
Hu C, Ni D, Nam KH, Majumdar S, McLean J, Stahlberg H, Terns MP, Ke A
Mol Cell (2022)
PMID: 35835111
Related structures: EMD-26082

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptadecamer (preferred)
PDBe Complex ID:
PDB-CPX-105345 (preferred)
Entry contents:
6 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (7 distinct):
CRISPR-associated helicase Cas3 Chain: A
Molecule details ›
Chain: A
Length: 572 amino acids
Theoretical weight: 61.41 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A5C0XNV5 (Residues: 9-450, 451-516; Coverage: 77%)
Gene names: PFDSM3638_03195, cas3
Sequence domains:
Type I-A CRISPR-associated protein Cas8a2/Csa4 Chain: C
Molecule details ›
Chain: C
Length: 341 amino acids
Theoretical weight: 38.8 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U338 (Residues: 3-346; Coverage: 98%)
Gene name: PF0637
Sequence domains: CRISPR-associated protein (Cas_Csa4)
Cas11a Chains: D, E, F, G, H
Type I-A CRISPR-associated protein Cas7/Csa2 Chains: I, J, K, L, M, N, O
Type I-A CRISPR-associated protein Cas5 Chain: P
CRISPR-associated endonuclease Cas3-HD Chain: Q
crRNA (45-MER) Chain: R

Ligands and Environments

1 bound ligand:
Ligand NI 2 x NI
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.6Å
Relevant EMDB volumes: EMD-26082
Expression system: Escherichia coli

Quick links

Citations

7 review citations

CRISPR-based nucleic acid diagnostics for pathogens.
Yang et al. (2023)
6 more

2 mentions without citation

Allosteric control of type I-A CRISPR-Cas3 complexes and establishment as effective nucleic acid detection and human genome editing tools.
Hu et al. (2022)
1 more