› 7trv

X-ray diffraction
1.8Å resolution

Crystal Structure of the DNA-Binding Domain of the LysR family Transcriptional Regulator YfbA from Yersinia pestis

Released:
DOI: 10.2210/pdb7trv/pdb
PDB entry 7trv coloured by chain, front view.
PDB entry 7trv coloured by chain, side view.
PDB entry 7trv coloured by chain, top view.
Source organism: Yersinia pestis CO92
Entry authors: Kim Y, Tesar C, Crawford M, Chhor G, Endres M, Babnigg G, Schneewind O, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104690 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
LysR-family transcriptional regulatory protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 90 amino acids
Theoretical weight: 10.37 KDa
Source organism: Yersinia pestis CO92
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A380PDL3 (Residues: 1-87; Coverage: 29%)
Gene name: YPO2150
Sequence domains: Bacterial regulatory helix-turn-helix protein, lysR family

Ligands and Environments

4 bound ligands:
Ligand SO4 6 x SO4
Ligand CL 8 x CL
Ligand GOL 3 x GOL
Ligand FMT 3 x FMT
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 30.943Å b: 146.27Å c: 33.701Å
α: 90° β: 90.205° γ: 90°
R-values:
R R work R free
0.196 0.187 0.232
Expression system: Escherichia coli BL21(DE3)

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